Salt and sugar levels for effective plant regeneration from in vitro cultured tissues of cocoyam

Abstract

Tips from apical and axillary meristems of cocoyam were grown in modified Murashige and Skoog's (M S) medium with a range of concentrations of salts and sucrose. Sixteen treatments were evaluated for their effect on root and shoot formation and plantlet development. The whole regeneration cycle from meristem tip to plantlet was obtained in a single medium (one step) system. Multiple regeneration was favored by high sugar-reduced salt combinations. The best results were obtained with combinations containing 133% of the sugar and 80% of the salt concentration in the unmodified M S medium. Better regeneration was obtained from axillary meristems than from apical.