Purification, Electron Microscopy, and Serology of Dioscorea Latent Virus

How to Cite

Waterworth, H. E., Lawson, R. H., & Kahn, R. P. (1974). Purification, Electron Microscopy, and Serology of Dioscorea Latent Virus. The Journal of Agriculture of the University of Puerto Rico, 58(3), 351–357. https://doi.org/10.46429/jaupr.v58i3.10670


A virus latent in yam species Dioscorea floribunda and D. composita (DLV) was purified by use of powdered activated charcoal and magnesium-treated bentonite to clarify extracted Dioscorea leaf sap. The virus produced a single band in sucrose-density gradients. Antisera produced in a rabbit reached a titer of 1:16,000. Purified DLV did not react with antiscra to any of 25 rod-shaped viruses. Myzus persicae (Sulzer), the green peach aphid, did not transmit DLV from infected Dioscorea plants. Seventy-four percent of the particles measured 395-445 nm. Inoculated D. floribunda seedlings showed no symptoms, but the virus was observed in dip preparations from systemically infected growth. DLV occurred in combination with a second virus that was aphid-transmissible and which incited green banding symptoms.


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