TY - JOUR AU - Fernández-Coll, Fred AU - Silva-Negrón, Luis PY - 1989/07/01 Y2 - 2024/03/28 TI - Conventional versus the petrifilm method for the enumeration of different types of bacteria JF - The Journal of Agriculture of the University of Puerto Rico JA - JAUPR VL - 73 IS - 3 SE - Articles DO - 10.46429/jaupr.v73i3.6455 UR - https://revistas.upr.edu/index.php/jaupr/article/view/6455 SP - 183-187 AB - <p>Performance of Petrifilm VRB and SM plates to enumerate different types of bacterial populations was evaluated. A total of 100 Petrifilms and 100 regular Petri plates were tested with each of 3 different bacterial populations in 4 experiments. The Petrifilm system was superior to the conventional pour plate methods, or at least not statistically different, in enumerating <em>Escherichia coli</em> (coliform) and a mixture of <em>E. coli</em>,<em> Staphylococcus aureus</em> and <em>Serratia marcescens</em> (total aerobic plate count). The Petrifilm method was superior to conventional plate counts in enumerating <em>Pseudomonas aeruginosa</em> (psychrotrophic plate count), when both incubations were at 21°-22° C for 96 h. If sustained in further experimentation, this last observation would mean that the Petrifilm SM system could be successfully used to detect bacterial populations in addition to those for which the system was originally intended.</p> ER -