Management of stockplant for the in vitro culture of nodal segments of guava (Psidium guajava L.).
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Ramírez-Villalobos, M., León de Sierralta, S., Marín, M., & Nava, A. (2004). Management of stockplant for the in vitro culture of nodal segments of guava (Psidium guajava L.). The Journal of Agriculture of the University of Puerto Rico, 88(1-2), 73–89. https://doi.org/10.46429/jaupr.v88i1-2.2736

Abstract

Nodal segments collected from shoots of adult grafts on juvenile rootstocks from seedlings grown under full sun and shade (40%), and from adult plants grown without and with shade (80%) were cultivated in vitro. In addition, nodal segments from field grown plants were cultivated in vitro under the following conditions: control; sixty days after dormex (hydrogenated cyanamide 49%) application at 2% and 4%; sixty days after partial pruning; and 75 days of 80% shading. Sixty days after culture initiation, seedlings and adult plants under shade produced no browning explants. The highest percentage of budbreak (PBB) (100%) was obtained at 20 days in explants of seedlings; at 40 days in explants of adult plants under shade. At sixty days, adult and grafted plants without shade presented 40% and 35% browning explants, respectively, and 85% and 80% budbreak explants, respectively. At sixty days, the control treatment produced 65% budbreak; the dormex treatment with the concentrations of 2% and 4%, 80% and 70% PBB, respectively; the partial pruning, 65%. Dormex application at 2% increased PBB and percentage of leaves formed to more than that of the control.
https://doi.org/10.46429/jaupr.v88i1-2.2736
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