Prevalence of Burkholderia glumae in rice crops in Ecuador
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Keywords

antibiotic sensitivity
bacterial panicle blight
pathogenicity assays
serological and molecular diagnosis

How to Cite

Paz-Carrasco, L. C., Intriago-Mendoza, L. D., Basso, M. F., & Celi-Herán, R. E. (2018). Prevalence of Burkholderia glumae in rice crops in Ecuador. The Journal of Agriculture of the University of Puerto Rico, 102(1-2), 65-78. Retrieved from https://revistas.upr.edu/index.php/jaupr/article/view/17532

Abstract

Burkholderia glumae is the agent responsible for bacterial panicle blight disease (BPBD) of rice that causes severe damage to this crop worldwide. During 2012 and 2013, symptoms of BPBD were observed in Palestina city, located in Guayas province, Ecuador. In 2014, the presence of B. glumae was confirmed at this location. In view of the socio-economic importance of rice in Ecuador, this research aimed to investigate the prevalence of B. glumae in other rice-producing regions. Eighteen bacterial isolates obtained from blighted kernels were characterized. Physiological, biochemical, serological, and molecular assays and the amplification of the 16S-23S rRNA ITS of the bacterial isolates collected confirmed the identity of the BPBD-associated bacterium. Pathogenicity assays verified the ability of these isolates to produce discoloration, spotting, and empty grains, symptoms associated with BPBD. Antibiotic assays showed that EC-EELS-01 isolate was sensitive to ciprofloxacin and tetracycline, and resistant to polymyxin. The dissemination and prevalence of B. glumae were confirmed in the rice-producing areas of El Oro, Cañar, Guayas, and Los Rios provinces. This research will serve to develop genetic studies to characterize the population in the B. glumae isolates in Ecuador.

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