Nature of the Vitamin A Activity of Annatto Seed (Bixa orellana L.)
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Santamaría, L., Martínez, M. L., & Asenjo, C. F. (1965). Nature of the Vitamin A Activity of Annatto Seed (Bixa orellana L.). The Journal of Agriculture of the University of Puerto Rico, 49(2), 259-268. https://doi.org/10.46429/jaupr.v49i2.13025

Abstract

The high vitamin A activity of the petroleum ether extract of the annatto seed has been confirmed. It ranges from 1,000 to 2,000 international units of vitamin A per gram of extract. It was found that a standard mixture of 3-percent acetone in petroleum ether did not remove any vitamin A-active material from a magnesium oxide-hyflo supercel column, on which a petroleum ether extract of annatto had been chromatographed. The only solvent among those tried, that effectively removed the material which was left absorbed on the column after elution with acetone-petroleum ether was methanol. The methanol extract exhibited a biological activity of about the same order of magnitude as the original petroleum ether extract, that is, 1,000 to 2,000 international units of vitamin A.
https://doi.org/10.46429/jaupr.v49i2.13025
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