Abstract
The effect of neem extracts on feeding behavior of Aphis nerii, as well as Papaya Ringspot Virus (PRSV-p) transmission, was determined. We evaluated papaya seedlings sprayed under laboratory conditions with a 10% aqueous extract of unpeeled neem seed and with a water control. Two time periods, exploratory (0 to 3 min) and feeding (>3 to 20 min), were used to evaluate aphid feeding behavior. Aphid mortality was determined 24 h after spraying. Viral transmission was measured by ELISA tests. No significant differences (p > 0.05) were obtained in the exploratory feeding behavior. However, significantly more aphids (p < 0.05) stopped feeding on neemsprayed plants from the >3 to 20 min period. Also, higher (p < 0.05) aphid mortality (37% vs. 10%) was found in neem-sprayed plants. However, no significant differences (p > 0.05) were found in PRSV-p transmission, thus indicating that neem seed aqueous extracts did not prevent viral transmission.